Product Description
This product is a sterile, low-endotoxin density gradient separation medium designed for isolating lymphocytes from rat bone marrow. The separation principle relies on cell density differences cells are distributed along corresponding density gradients via centrifugation, thereby enabling lymphocyte isolation. It is suitable for isolating lymphocytes from bone marrow, and lymphocytes obtained under sterile conditions can be used for cell culture. This kit ensures >80% recovery and purity of target cells. For higher-purity T cell or B cell populations, additional immunomagnetic bead-based separation is recommended. This kit can reduce magnetic bead consumption and lower costs.
Transport and Storage
| Parameter | Specification | Important Notes |
|---|---|---|
| Transport | Room temperature | - |
| Storage | 15-25°C, protected from light | DO NOT refrigerate or freeze. Storage at 4°C may cause white crystallization, compromising separation performance. |
| Handling | All operations must be performed under sterile conditions. | - |
Precautions
Use appropriate consumables: Avoid high-polymer materials (e.g., polystyrene) due to static electricity. Use static-free or low-static centrifuge tubes and non-alkali-treated glassware, as static causes cell adhesion to tube walls and alkali-treated glass surfaces become roughened, compromising separation efficiency.
Avoid plasma contamination: Aspirating excessive material above the target cell layer will introduce plasma proteins and platelets.
Proper dilution is critical: Improper dilution reduces cell yield and viability. Diluent requirements：Calcium/magnesium-free buffer or medium. If blood is diluted, reduce centrifugal force and time accordingly.
