Product Description
This product is a sterile, low-endotoxin density gradient separation medium designed for isolating lymphocytes from mouse spleen tissue. The separation principle relies on density differences among cells, where centrifugation distributes cells according to their respective densities, thereby separating lymphocytes from spleen tissue. Lymphocytes isolated under sterile conditions are suitable for cell culture applications. This kit ensures a target cell recovery rate and purity of >80%. For higher purity T cells or B cells, combine with immunomagnetic bead sorting. This separation solution reduces magnetic bead consumption and associated costs.
Transport and Storage
| Parameter | Specification | Important Notes |
|---|---|---|
| Transport | Room temperature | - |
| Storage | 15-25°C, protected from light | DO NOT refrigerate or freeze. Storage at 4°C may cause white crystallization, compromising separation performance. |
| Handling | All operations must be performed under sterile conditions. | - |
Precautions
Use appropriate consumables: Avoid high-polymer materials (e.g., polystyrene) due to static electricity. Use static-free or low-static centrifuge tubes and non-alkali-treated glassware, as static causes cell adhesion to tube walls and alkali-treated glass surfaces become roughened, compromising separation efficiency.
Avoid plasma contamination: Aspirating excessive material above the target cell layer will introduce plasma proteins and platelets.
Proper dilution is critical: Improper dilution reduces cell yield and viability. Diluent requirements：Calcium/magnesium-free buffer or medium. If blood is diluted, reduce centrifugal force and time accordingly.
