Product Description
This product isolates CD4+ T cells from human peripheral blood mononuclear cells (PBMC) via negative selection. The principle is to label non-target cells (non-CD4+ T cells) with biotin-conjugated monoclonal antibodies, followed by depletion with streptavidin-conjugated magnetic beads, thereby achieving purification of human CD4+ T cells. A magnetic stand is required for the separation process.
Storage Conditions and Shelf Life
Storage Conditions: Store at 2–8°C. DO NOT FREEZE.
Shelf Life: 2 years
Applications
This kit is suitable for isolating CD4+ T cells from freshly isolated or frozen human PBMC.
Precautions
Avoid freezing magnetic beads and antibody mix during storage and use.
Use low-retention pipette tips and centrifuge tubes to minimize loss of beads and antibodies due to adsorption.
This product should be used with a magnetic separator.
For research use only.
